The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 14, 2021
Filed:
Dec. 10, 2020
Nanjing Tech University, Jiangsu, CN;
Hanjie Ying, Jiangsu, CN;
Dong Liu, Jiangsu, CN;
Yong Chen, Jiangsu, CN;
Qingguo Liu, Jiangsu, CN;
Pingkai Ouyang, Jiangsu, CN;
Ming Lei, Jiangsu, CN;
Huanqing Niu, Jiangsu, CN;
Bin Yu, Jiangsu, CN;
Di Zhang, Jiangsu, CN;
Yuhan Yang, Jiangsu, CN;
Shishi Wu, Jiangsu, CN;
Nanjing Tech University, Jiangsu, CN;
Abstract
The invention discloses a recombinantfor producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinantis constructed by overexpressing a protease gene FtsH in a; and the construction method comprises the following steps of: (1) performing PCR on a genome of the, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the, and screening to obtain the recombinant. The invention has the beneficial effects that thefor overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of theis enhanced, such that a yield of continuous immobilized fermentation of theis increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.