The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 14, 2021
Filed:
Jul. 14, 2017
Applicant:
Wisconsin Alumni Research Foundation, Madison, WI (US);
Inventors:
James A. Thomson, Madison, WI (US);
Srikumar Sengupta, Madison, WI (US);
Assignee:
Wisconsin Alumni Research Foundation, Madison, WI (US);
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/407 (2015.01); C12N 5/071 (2010.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01); C12N 5/00 (2006.01); C12Q 1/00 (2006.01);
U.S. Cl.
CPC ...
A61K 35/407 (2013.01); C12N 5/067 (2013.01); C12Q 1/02 (2013.01); G01N 33/50 (2013.01); C12N 5/00 (2013.01); C12N 2501/119 (2013.01); C12N 2501/12 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/237 (2013.01); C12N 2501/39 (2013.01); C12N 2501/42 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2503/02 (2013.01); C12N 2506/02 (2013.01); C12Q 1/00 (2013.01);
Abstract
This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.