The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 07, 2021

Filed:

Dec. 27, 2017
Applicants:

Chiyoda Corporation, Kanagawa, JP;

Confocal Science Inc., Tokyo, JP;

Inventors:

Naoki Tanigawa, Kanagawa, JP;

Hiroaki Tanaka, Tokyo, JP;

Sachiko Takahashi, Tokyo, JP;

Koji Inaka, Nara, JP;

Assignees:

CHIYODA CORPORATION, Kanagawa, JP;

CONFOCAL SCIENCE INC., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C30B 29/58 (2006.01); B01D 9/00 (2006.01); B01D 61/36 (2006.01); C12N 9/36 (2006.01);
U.S. Cl.
CPC ...
B01D 9/005 (2013.01); B01D 9/0077 (2013.01); B01D 61/362 (2013.01); C12N 9/2462 (2013.01); C12Y 302/01017 (2013.01); B01D 2009/0086 (2013.01); B01D 2311/12 (2013.01); B01D 2311/2642 (2013.01);
Abstract

Provided are: an economically superior protein crystallization method capable of efficiently finding conditions for crystallization by using a small amount of protein; and a crystallization device used for the method. According to the present invention, a transparent sealed containeris filled with a solution of protein, a part of the transparent sealed containerbeing formed of a semipermeable membranewith a molecular weight cut-off that inhibits passage of the protein while allowing passage of a precipitant, and then, a precipitant solution with changed concentration and/or pH of the precipitant is continuously supplied to the semipermeable membrane, to crystallize the protein with the precipitant that infiltrates from the semipermeable membraneinto the sealed container


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