The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 31, 2021
Filed:
Jan. 28, 2019
Berkeley Lights, Inc., Emeryville, CA (US);
Gregory G. Lavieu, Vitry sur Seine, FR;
Annamaria Mocciaro, San Francisco, CA (US);
Xiao Guan Radstrom, San Rafael, CA (US);
Jason M. McEwen, El Cerrito, CA (US);
Magali Soumillon, Berkeley, CA (US);
J. Tanner Nevill, El Cerrito, CA (US);
Volker L. S. Kurz, Oakland, CA (US);
Patricia A. Dyck, San Francisco, CA (US);
Ravi K. Ramenani, Fremont, CA (US);
Berkeley Lights, Inc., Emeryville, CA (US);
Abstract
Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.