The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Jul. 02, 2014
Applicants:

Vib Vzw, Ghent, BE;

Universiteit Gent, Ghent, BE;

Inventors:

Nico L. M. Callewaert, Nevele, BE;

Karen De Pourcq, Ghent, BE;

Steven Geysens, Wannegem-Lede, BE;

Leander Meuris, Ghent, BE;

Assignees:

VIB VZW, Ghent, BE;

Universiteit Gent, Ghent, BE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 9/24 (2006.01); C12P 21/00 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01096 (2013.01); C12Y 302/01113 (2013.01); C07K 2319/02 (2013.01);
Abstract

The disclosure relates to the field of glyco-engineering, more specifically, to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells are glyco-engineered yeast cells in which, additionally, at least one exogenous enzyme needed for complex glycosylation is present, e.g., allowing easier separation of differentially glycosylated glycoproteins.


Find Patent Forward Citations

Loading…