The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Apr. 30, 2021
Applicant:

New England Biolabs, Inc., Ipswich, MA (US);

Inventors:

Mehul Ganatra, Gloucester, MA (US);

Siu-Hong Chan, Ipswich, MA (US);

Christopher H. Taron, Essex, MA (US);

G. B. Robb, Somerville, MA (US);

Assignee:

New England Biolabs, Inc., Ipswich, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01); C12N 9/12 (2006.01); C12N 7/00 (2006.01); C12N 9/10 (2006.01); A61K 39/245 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/16 (2013.01); A61K 39/245 (2013.01); C12N 7/00 (2013.01); C12N 9/1007 (2013.01); C12N 9/1241 (2013.01); C12Y 201/01056 (2013.01); C12Y 207/0705 (2013.01); C12Y 301/03033 (2013.01); A61K 2039/53 (2013.01); C07K 2319/21 (2013.01); C12N 2710/16134 (2013.01);
Abstract

The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.


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