The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Nov. 11, 2020
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Zhiming Rao, Wuxi, CN;

Minglong Shao, Wuxi, CN;

Yetong Wang, Wuxi, CN;

Yuling Wu, Wuxi, CN;

Taowei Yang, Wuxi, CN;

Xian Zhang, Wuxi, CN;

Meijuan Xu, Wuxi, CN;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/04 (2006.01); C12N 1/20 (2006.01); C12N 15/70 (2006.01); C12N 15/52 (2006.01); C12P 33/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0006 (2013.01); C12N 1/20 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12P 33/02 (2013.01); C12Y 101/01051 (2013.01);
Abstract

The disclosure discloses 17β-hydroxysteroid dehydrogenase mutants and application thereof, and belongs to the technical field of biology. The disclosure provides 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y with high specific enzyme activities, and the specific enzyme activities of the 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y are as high as 1.85, 1.93, 2.06 and 5.15 U/mg, respectively, which are 1.11, 1.16, 1.24 and 3.10 times larger than that of wild-type 17β-hydroxysteroid dehydrogenase (1.66 U/mg).


Find Patent Forward Citations

Loading…