The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

Feb. 20, 2017
Applicant:

Versalis S.p.a., San Donato Milanese, IT;

Inventor:

Fabio Sabbatini, Siena, IT;

Assignee:

Versalis S.p.A, San Donato Milanese, IT;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/36 (2006.01); C12N 1/16 (2006.01); C12P 1/02 (2006.01); C12N 1/22 (2006.01); C07K 14/40 (2006.01); C12N 1/18 (2006.01);
U.S. Cl.
CPC ...
C12N 1/22 (2013.01); C07K 14/40 (2013.01); C12N 1/16 (2013.01); C12N 1/18 (2013.01); C12N 1/36 (2013.01); C12P 1/02 (2013.01);
Abstract

It is disclosed a process for propagating a yeast capable to ferment glucose and xylose of a lignocellulosic feedstock hydrolyzate, said process comprising propagating the yeast over at least two propagation cycles. The first propagation cycle comprises the steps of: contacting the yeast at a starting yeast density with a first cultivation medium comprising a first portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a first populated broth comprising water and a first propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the first cultivation medium are consumed in the first propagation cycle. The second cycle comprises the steps of: separating the first populated broth in at least a first removed portion and a first residual portion, wherein both the first residual portion and the first removed portion comprise some of the first propagated yeast; contacting the first residual portion with a second cultivation medium comprising a second portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a second populated broth comprising water and a second propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the second cultivation medium are consumed in the second propagation cycle.


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