The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 03, 2021

Filed:

Apr. 11, 2018
Applicants:

National Institute of Advanced Industrial Science and Technology, Tokyo, JP;

Nissan Chemical Corporation, Tokyo, JP;

National University Corporation Kyoto Institute of Technology, Kyoto, JP;

Inventors:

Hiroaki Tateno, Ibaraki, JP;

Junko Katayama, Tokyo, JP;

Kazutaka Matoba, Chiba, JP;

Yoichi Kumada, Kyoto, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 7/06 (2006.01); C07K 14/195 (2006.01); C07K 17/08 (2006.01); C07K 17/14 (2006.01); C07K 19/00 (2006.01); G01N 33/545 (2006.01); G01N 33/551 (2006.01); G01N 33/566 (2006.01); C07K 14/705 (2006.01); C07K 17/00 (2006.01); C12N 15/62 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
C07K 14/7056 (2013.01); C07K 7/06 (2013.01); C07K 14/195 (2013.01); C07K 17/00 (2013.01); C07K 17/08 (2013.01); C07K 17/14 (2013.01); C12N 15/62 (2013.01); G01N 33/543 (2013.01); G01N 33/545 (2013.01); G01N 33/54353 (2013.01); G01N 33/551 (2013.01); G01N 33/566 (2013.01); C07K 2319/00 (2013.01); C07K 2319/20 (2013.01); C07K 2319/40 (2013.01);
Abstract

Provided is a highly sensitive and less expensive lectin-immobilized base material (for example, a lectin plate), such as lectin-immobilized base material having stable qualities and being able to be sufficiently washed after a target sugar chain-containing antigen binds thereto. Further provided is a method for immobilizing lectin to a base material therefor. Particularly provided are: a method whereby a lectin-peptide fusion, in which a peptide capable of adsorbing to a base material surface such as a polystyrene (PS) tag is fused with the N-terminal side or C-terminal side of lectin capable of recognizing a target sugar chain, is immobilized on the peptide side to a base material; and a lectin-immobilized base material produced by this method. By using the lectin-immobilized base material, a target sugar chain-containing antigen can be highly sensitively and evenly measured and, moreover, target sugar chain-containing cells, etc. can be separated (concentrated and harvested).


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