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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11060123 B1

PDFFull Text
Date of Patent:
Jul. 13, 2021

Filed:

Mar. 16, 2020

Production of soluble recombinant protein without n-terminal methionine

Applicant:

Fina Biosolutions, Llc, Rockville, MD (US);

Inventors:

Natalia Oganesyan, North Potomac, MD (US);

Andrew Lees, Silver Spring, MD (US);

Min-Ju Chang, Gaithersburg, MD (US);

Assignee:

Fina BioSolutions, LLC, Rockville, MD (US);

Attorney:

Remenick PLLC

Primary Examiner:

Gary B Nickol

Assistant Examiner:

Lakia J Jackson-Tongue

Int. Cl.
CPC ...
A61K 39/00 (2006.01); A61K 39/385 (2006.01); A61K 39/02 (2006.01); A61K 39/40 (2006.01); A61K 48/00 (2006.01); A01N 63/00 (2020.01); C12P 21/06 (2006.01); C12P 21/02 (2006.01); C07K 14/34 (2006.01); C12N 15/70 (2006.01); C07K 1/16 (2006.01); C12N 1/20 (2006.01); C12R 1/19 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 1/16 (2013.01); C07K 14/34 (2013.01); C12N 15/70 (2013.01); C07K 2319/00 (2013.01); C12N 1/205 (2021.05); C12R 2001/19 (2021.05);
Abstract

The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5'-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRMprotein from. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRMas well as characterization of properly folded CRMprotein.

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