The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 13, 2021
Filed:
Nov. 13, 2019
Jiangnan University, Jiangsu, CN;
Zhiming Rao, Jiangsu, CN;
Song Liu, Jiangsu, CN;
Renjie Gao, Jiangsu, CN;
Xian Zhang, Jiangsu, CN;
Taowei Yang, Jiangsu, CN;
Meijuan Xu, Jiangsu, CN;
JIANGNAN UNIVERSITY, Jiangsu, CN;
Abstract
The present invention discloses a method for producing a 1,2-amino alcohol compound by utilizing whole-cell transformation, and belongs to the technical field of gene engineering and microorganism engineering. According to the present invention, engineeredco-expresses epoxide hydrolase, alcohol dehydrogenase, ω-transaminase and glutamate dehydrogenase, is capable of realizing whole-cell catalysis of an epoxide in one step to synthesize a 1,2-amino alcohol compound, and meanwhile, can realize regeneration of coenzyme NADPand an amino doner L-Glu; alcohol dehydrogenase expressed by the engineeredis RBS optimized alcohol dehydrogenase, and such RBS optimization can control the expression quantity of alcohol dehydrogenase, so that the catalysis rate of alcohol dehydrogenase and transaminase can achieve an optimum ratio, to eliminate influence caused by a rate-limiting step in a catalyzing course.