The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 15, 2021

Filed:

Jun. 28, 2018
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Daniel Branton, Lexington, MA (US);

Stephen Jordan Fleming, Cambridge, MA (US);

Jene A. Golovchenko, Lexington, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/487 (2006.01); B01D 71/02 (2006.01); B01D 71/74 (2006.01); C12Q 1/6869 (2018.01); G01N 27/40 (2006.01); B82Y 35/00 (2011.01);
U.S. Cl.
CPC ...
G01N 33/48721 (2013.01); B01D 71/021 (2013.01); B01D 71/74 (2013.01); C12Q 1/6869 (2013.01); G01N 27/40 (2013.01); B82Y 35/00 (2013.01); C12Y 306/01039 (2013.01);
Abstract

In a method p for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound to a sequential plurality of polymer subunits along the target polymer molecule length and the molecule and clamp are disposed in an ionic solution that is in fluidic communication with the nanopore. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore, until the clamp abuts the nanopore aperture and stops further travel of the target polymer molecule into the nanopore. Then a voltage control pulse is applied across the nanopore and/or a thermal control pulse is applied at the nanopore, with a pulse duration that steps the clamp along the target polymer molecule by no more than one polymer subunit in a direction opposite that of travel into the nanopore. No fuel is provided to the clamp.


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