The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11021724 B1

PDF
Full Text
Expired
Date of Patent:
Jun. 01, 2021

Filed:

Mar. 29, 2018
Applicants:

University of Florida Research Foundation, Inc., Gainesville, FL (US);

Agroparistech, Paris, FR;

Inventors:

Jon Dale Stewart, Orlando, FL (US);

Florent Allais, Bouy, FR;

Louis Michel Marie Mouterde, Reims, FR;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/18 (2006.01);
U.S. Cl.
CPC ...
C12P 17/181 (2013.01); C12Y 106/99001 (2013.01);
Abstract

The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (S)-γ-hydroxymethyl-α,β-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 fromand comprises the amino acid sequence of SEQ ID NO: I. In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).


Find Patent Forward Citations

Stewart, Jon. (2021). Materials and methods for alkene reduction of levoglucosenone by an alkene reductase (U.S. Patent No. 11021724). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11021724

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