The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 04, 2021
Filed:
Jan. 22, 2018
Massachusetts Institute of Technology, Cambridge, MA (US);
Fei Chen, Cambridge, MA (US);
Asmamaw T. Wassie, Boston, MA (US);
Shahar Alon, Cambridge, MA (US);
Adam Henry Marblestone, Arlington, MA (US);
Anubhav Sinha, Cambridge, MA (US);
Andrew Payne, Cambridge, MA (US);
Edward Stuart Boyden, Chestnut Hill, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Abstract
The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.