The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 27, 2021
Filed:
Sep. 30, 2016
Symrise Ag, Holzminden, DE;
Leibniz-institut Für Pflanzenbiochemie, Halle, DE;
Ludger A. Wessjohann, Halle, DE;
Susann Herrmann, Halle, DE;
Martin Dippe, Halle, DE;
Torsten Geissler, Einbeck, DE;
Katrin Geissler, Einbeck, DE;
Jakob Peter Ley, Holzminden, DE;
SYMRISE AG, Holzminden, DE;
LEIBNIZ-INSTITUT FÜR PFLANZENBIOCHEMIE, Halle, DE;
Abstract
The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.