The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 27, 2021
Filed:
Dec. 28, 2016
Beijing Minhai Biotechnology Co., Ltd., Beijing, CN;
Guoshun Li, Beijing, CN;
Meirong Gu, Beijing, CN;
Jiankai Liu, Beijing, CN;
Lin Guo, Beijing, CN;
Lei Chen, Beijing, CN;
Gaimei Zhang, Beijing, CN;
Yingzhi Xu, Beijing, CN;
Jin Li, Beijing, CN;
Haifeng Xiao, Beijing, CN;
Beijing Minhai Biotechnology Co., Ltd., Beijing, CN;
Abstract
The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria; inducing expression of the EV71 virus-like particle protein expression using methanol; collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption; performing precipitation on the supernatant with ammonium sulfate; and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.