The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 13, 2021

Filed:

Jul. 15, 2015
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Joseph M. Jacobson, Newton, MA (US);

Noah Michael Jakimo, Boston, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C07K 19/00 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 9/22 (2013.01); C07K 2319/00 (2013.01); C07K 2319/81 (2013.01); C12N 2310/20 (2017.05);
Abstract

The invention provides compositions and methods for repeatable directed endonucleases (RDEs) and methods for repeatedly, and specifically cleaving DNA offset from the RDE's DNA recognition sequence on the target nucleic acid rather than within the DNA recognition sequence. Conservation of the recognition sequence of the target nucleic acid enables for re-localization of an RDE back to the DNA recognition sequence for further cleavage. The RDEs and methods of the invention are useful in applications including, but not limited to, recording data into a genome, timing the order of biochemical pathway events, efficient genome engineering and encoding lagged cellular death.


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