The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 13, 2021
Filed:
Jul. 10, 2018
Applicant:
Twist Bioscience Corporation, San Francisco, CA (US);
Inventors:
Anthony Cox, Mountain View, CA (US);
Siyuan Chen, San Mateo, CA (US);
Assignee:
TWIST BIOSCIENCE CORPORATION, San Francisco, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C40B 50/14 (2006.01); C12Q 1/6816 (2018.01); C40B 40/06 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1068 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12N 15/111 (2013.01); C12N 2310/10 (2013.01); C12N 2310/20 (2017.05); C12N 2330/31 (2013.01); C40B 40/06 (2013.01); C40B 50/14 (2013.01);
Abstract
Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.