The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 2021

Filed:

Sep. 26, 2017
Applicants:

Riken, Wako, JP;

Zeon Corporation, Tokyo, JP;

The Yokohama Rubber Co., Ltd., Tokyo, JP;

Inventors:

Ryoko Orishimo, Wako, JP;

Tomokazu Shirai, Wako, JP;

Kazuhiro Takahashi, Tokyo, JP;

Misao Hiza, Hiratsuka, JP;

Yusuke Tanabe, Hiratsuka, JP;

Assignees:

RIKEN, Wako, JP;

ZEON CORPORATION, Tokyo, JP;

THE YOKOHAMA RUBBER CO., LTD., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 5/00 (2006.01); C12P 5/02 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C12N 15/82 (2006.01); C12N 15/09 (2006.01); C12P 21/02 (2006.01); C12N 5/10 (2006.01);
U.S. Cl.
CPC ...
C12P 5/007 (2013.01); C12N 5/10 (2013.01); C12N 9/88 (2013.01); C12N 15/09 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12N 15/743 (2013.01); C12N 15/8205 (2013.01); C12N 15/8257 (2013.01); C12P 5/02 (2013.01); C12P 5/026 (2013.01); C12P 21/02 (2013.01); C12Y 401/01033 (2013.01);
Abstract

Mutations involving amino acid substitution were introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Then, the variants were each evaluated in terms of a catalytic activity for production of olefin compounds such as isoprene. As a result, it was found that substitution of glycine at position with a different amino acid resulted in improvement in the catalytic activity. In addition, it was found that the MVD in which arginine at position and threonine at position in addition to the position were further substituted with different amino acids, respectively, also had the high catalytic activity.


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