The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 16, 2021

Filed:

Nov. 10, 2016
Applicant:

Pfizer Inc., New York, NY (US);

Inventors:

Sunil Gururao Desai, Andover, MA (US);

Michael Allen Hanson, Pearl River, NY (US);

Jonathan Patrick Kinross, Reading, MA (US);

Daniel R. Lasko, Medford, MA (US);

Scott Ellis Lomberk, Suffern, NY (US);

Jason Arnold Lotvin, West Nyack, NY (US);

Sujata Kaushikbhai Patel-Brown, Valley Cottage, NY (US);

Weiqiang Sun, Morristown, NJ (US);

Peter Anthony Tomasello, Methuen, MA (US);

Assignee:

Pfizer Inc., New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12P 19/04 (2006.01); C12R 1/46 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C12P 19/04 (2013.01); C12R 1/46 (2013.01);
Abstract

The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.


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