The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

Nov. 02, 2015
Applicant:

National University Corporation Kobe University, Kobe, JP;

Inventors:

Keiji Nishida, Kobe, JP;

Akihiko Kondo, Kobe, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C07K 19/00 (2006.01); C12N 9/24 (2006.01); C12N 15/09 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1024 (2013.01); C07K 19/00 (2013.01); C12N 9/22 (2013.01); C12N 9/24 (2013.01); C12N 9/2497 (2013.01); C12N 15/09 (2013.01); C12N 15/11 (2013.01); C12Y 302/02027 (2013.01); C12N 2310/20 (2017.05);
Abstract

The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.


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