The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 02, 2021

Filed:

Mar. 10, 2017
Applicant:

Dots Technology Corp., Natick, MA (US);

Inventors:

Adi Gilboa-Geffen, Wayland, MA (US);

Renuka Babu Brown, Weston, MA (US);

Sarah Stidham, Brookline, MA (US);

Valerie Villareal, Boston, MA (US);

Adam J. Young, Dedham, MA (US);

Joshua Glenn Anthony, Reading, MA (US);

Patrick Murphy, Cambridge, MA (US);

John H. Kepler, Lexington, MA (US);

Adam Jacobs, Hollis, NH (US);

Assignee:

DOTS TECHNOLOGY CORP., Natick, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01L 1/00 (2006.01); G01N 33/02 (2006.01); C12M 1/34 (2006.01); G01N 1/08 (2006.01); C12N 15/115 (2010.01); G01N 1/14 (2006.01); G01N 1/28 (2006.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/02 (2013.01); C12M 1/3476 (2013.01); C12N 15/115 (2013.01); G01N 1/08 (2013.01); G01N 1/14 (2013.01); G01N 1/286 (2013.01); G01N 33/5304 (2013.01); G01N 33/5308 (2013.01); G01N 33/58 (2013.01); C12N 2310/16 (2013.01); C12N 2320/11 (2013.01); G01N 2001/1427 (2013.01); G01N 2001/2866 (2013.01);
Abstract

The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.


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