The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 02, 2021
Filed:
May. 21, 2013
Src, Inc., North Syracuse, NY (US);
Huda Sirageldin Suliman, North Syracuse, NY (US);
Stacey Ann Massulik, Syracuse, NY (US);
Frances Louise Stites, Ashburn, VA (US);
Timothy Francis Moshier, Fulton, NY (US);
Kenton Arthur Doctor, East Syracuse, NY (US);
Jeffrey Harold Mills, Liverpool, NY (US);
Lisa Helen Chamberlin, East Syracuse, NY (US);
ACUMEN DETECTION, LLC, North Syracuse, NY (US);
Abstract
A device, method, and system for the detection of ribosome inactivating protein activity, including the ricin toxin, in a sample. According to one embodiment, the ribosome inactivating protein in the sample removes an adenine from a labeled DNA substrate to create an abasic site. An AP lyase can then cleave the DNA substrate at the abasic site, allowing the fluorophore located at or near one end of the DNA substrate and the quencher at or near the other end of the DNA substrate to spatially separate. Once the fluorophore and the quencher are sufficiently separated, the fluorophore will emit a fluorescence signal. Increasing fluorescence, indicating ribosome inactivating protein activity, will be monitored in real time using a detection system.