The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 02, 2021
Filed:
Jun. 14, 2018
Wisconsin Alumni Research Foundation, Madison, WI (US);
Jared Matthew Carlson-Stevermer, Madison, WI (US);
Krishanu Saha, Middleton, WI (US);
Amr Ashraf Abdeen, Madison, WI (US);
Lucille Katherine Kohlenberg, Madison, WI (US);
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US);
Abstract
Described herein are modified guide RNAs such as a single guide RNA including, from 5' to 3′, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.