The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2020
Filed:
Jun. 15, 2020
Jiangnan University, Jiangsu, CN;
Zhiming Rao, Jiangsu, CN;
Yaling Wang, Jiangsu, CN;
Jiamin Chen, Jiangsu, CN;
Taowei Yang, Jiangsu, CN;
Meijuan Xu, Jiangsu, CN;
Xian Zhang, Jiangsu, CN;
Minglong Shao, Jiangsu, CN;
Jianing Zhang, Jiangsu, CN;
Anqi Peng, Jiangsu, CN;
Shuping Xu, Jiangsu, CN;
Meiqi Wu, Jiangsu, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a β-amino acid, and providing a practical and effective strategy for industrial production of the β-amino acid.