The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 08, 2020
Filed:
Aug. 29, 2017
Applicant:
Integrated Dna Technologies, Inc., Coralville, IA (US);
Inventors:
Joseph A. Walder, Chicago, IL (US);
Joseph Dobosy, Coralville, IA (US);
Mark Aaron Behlke, Coralville, IA (US);
Assignee:
Integrated DNA Technologies, Inc., Coralville, IA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6853 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6869 (2013.01); C12N 2310/122 (2013.01);
Abstract
The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.