The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 08, 2020

Filed:

Jul. 01, 2016
Applicants:

Beihao Stem Cell and Regenerative Medicine Research Institutes Co., Ltd., Guangdong, CN;

Peking University, Beijing, CN;

Inventors:

Hongkui Deng, Beijing, CN;

Xiang Li, Beijing, CN;

Xiaohan Zuo, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); A61K 35/30 (2015.01); A61K 9/00 (2006.01); C12N 5/074 (2010.01); C12N 5/0793 (2010.01);
U.S. Cl.
CPC ...
A61K 35/30 (2013.01); A61K 9/0019 (2013.01); C12N 5/0607 (2013.01); C12N 5/0619 (2013.01); C12N 2501/01 (2013.01); C12N 2501/115 (2013.01); C12N 2501/13 (2013.01); C12N 2501/15 (2013.01); C12N 2501/60 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2502/086 (2013.01); C12N 2503/02 (2013.01); C12N 2506/08 (2013.01); C12N 2506/1307 (2013.01); C12N 2506/1353 (2013.01); C12N 2506/1369 (2013.01); C12N 2506/1384 (2013.01); C12N 2506/1392 (2013.01);
Abstract

Cocktails of chemical inducers of neuron-like properties (CINP) is provided, which includes cAMP agonists, neurogenic small molecules, glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, and BET family bromodomain inhibitors and optionally, a selective inhibitor of ROCK or p38 MAPK. These cocktails are used in a method of inducing neuron-like properties in partially or completely differentiated non-neuronal cells. The method includes contacting cells of a first type (non-neuronal) with the CINPs for a sufficient period of time to result in reprogramming the cell into cells of a second type having neuron-like characteristics (CiNs). Isolated chemically induced neurons (CiNs) can be used in a number of applications, including but not limited to cell therapy.


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