The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2020

Filed:

Sep. 16, 2016
Applicant:

Twist Bioscience Corporation, San Francisco, CA (US);

Inventors:

Anthony Cox, Mountain View, CA (US);

Sebastian Treusch, San Francisco, CA (US);

Siyuan Chen, San Mateo, CA (US);

Assignee:

Twist Bioscience Corporation, San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 50/06 (2006.01); C12N 15/10 (2006.01); G01N 33/50 (2006.01); C40B 50/14 (2006.01); C40B 50/08 (2006.01); C12N 15/113 (2010.01); C12Q 1/6876 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); C12N 15/1079 (2013.01); C12N 15/113 (2013.01); C12Q 1/6876 (2013.01); C40B 50/08 (2013.01); C40B 50/14 (2013.01); G01N 33/502 (2013.01); G01N 33/5029 (2013.01); G01N 33/5041 (2013.01); C12N 2320/30 (2013.01); C12N 2330/31 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/158 (2013.01); C40B 50/06 (2013.01);
Abstract

Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.


Find Patent Forward Citations

Loading…