The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2020

Filed:

Sep. 01, 2015
Applicant:

Institute of Biotechnology, Academy of Military Medical Sciences, China, Beijing, CN;

Inventors:

Jun Wu, Beijing, CN;

Chao Pan, Beijing, CN;

Peng Sun, Beijing, CN;

Hengliang Wang, Beijing, CN;

Bo Liu, Beijing, CN;

Zhehui Peng, Beijing, CN;

Li Zhu, Beijing, CN;

Shaohong Chang, Beijing, CN;

Xin Gong, Beijing, CN;

Erling Feng, Beijing, CN;

Bin Wang, Beijing, CN;

Ming Zeng, Beijing, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 39/40 (2006.01); C07K 14/33 (2006.01); A61K 39/08 (2006.01); A61K 39/104 (2006.01); C07K 19/00 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C07K 14/21 (2006.01); A61P 31/04 (2006.01);
U.S. Cl.
CPC ...
C07K 14/33 (2013.01); A61K 39/08 (2013.01); A61K 39/104 (2013.01); C07K 14/212 (2013.01); C07K 19/00 (2013.01); C12N 15/70 (2013.01); C12N 15/74 (2013.01); A61P 31/04 (2018.01); C07K 2319/55 (2013.01); C12Y 204/01 (2013.01); Y02A 50/30 (2018.01);
Abstract

The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and theO-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.


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