The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 13, 2020

Filed:

Feb. 09, 2015
Applicants:

Carl Zeiss Microscopy Gmbh, Jena, DE;

Carl Zeiss Ag, Oberkochen, DE;

Inventors:

Tiemo Anhut, Jena, DE;

Daniel Schwedt, Weimar, DE;

Matthias Wald, Jena, DE;

Assignees:

Carl Zeiss Microscopy GmbH, Jena, DE;

Carl Zeiss AG, Oberkochen, DE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G02B 21/00 (2006.01); G02B 21/36 (2006.01); G02B 27/00 (2006.01); G02B 21/33 (2006.01); G02B 27/09 (2006.01); G02B 27/10 (2006.01); G02B 27/42 (2006.01);
U.S. Cl.
CPC ...
G02B 21/0076 (2013.01); G02B 21/004 (2013.01); G02B 21/0032 (2013.01); G02B 21/0064 (2013.01); G02B 21/33 (2013.01); G02B 21/367 (2013.01); G02B 27/0037 (2013.01); G02B 27/0075 (2013.01); G02B 27/0927 (2013.01); G02B 27/0988 (2013.01); G02B 27/1086 (2013.01); G02B 27/4211 (2013.01); G02B 27/4227 (2013.01);
Abstract

Scanning fluorescence microscopes with an observation beam path from a measurement volume to an image plane. A beam combiner is provided for coupling an illumination system and a diaphragm arranged in the image plane for slow composition of the image because of the sequential scanning and subject the sample to loading as a result of inefficient use of the excitation light. The microscope simultaneously detects fluorescence from different focal planes in each case quasi-confocally. The observation beam path between the beam combiner and the image plane has a first diffractive optics for splitting light beams into beam bundles along different orders of diffraction, imparting to the light beams a spherical phase that is different from the other orders of diffraction. A second diffractive optics is provided for the compensation of chromatic aberrations of the split beam bundles, and a collecting optics is provided for focusing split beam bundles into the image plane.


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