The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 06, 2020

Filed:

Dec. 21, 2017
Applicants:

Kaneka Corporation, Osaka, JP;

Kyoto University, Kyoto, JP;

Inventors:

Tomohisa Kato, Hyogo, JP;

Kazuya Hamada, Hyogo, JP;

Haruhisa Inoue, Kyoto, JP;

Takayuki Kondo, Kyoto, JP;

Assignees:

KANEKA CORPORATION, Osaka, JP;

KYOTO UNIVERSITY, Kyoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/06 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); C12M 1/00 (2006.01);
U.S. Cl.
CPC ...
C12M 27/16 (2013.01); C12M 29/00 (2013.01); C12M 29/06 (2013.01); C12M 29/14 (2013.01); C12M 29/26 (2013.01); G01N 33/5032 (2013.01); G01N 33/5058 (2013.01); G01N 33/6896 (2013.01); G01N 2800/2835 (2013.01);
Abstract

A liquid injection method for injecting a liquid into a culture vessel includes tilting the culture vessel around a horizontal axis at a tilt angle (X°) of greater than 0° and 50° or less, wherein adherent cells are adhered to the culture vessel; and injecting the liquid into the culture vessel at a predetermined linear velocity (Y mm/s) via a wall surface of the culture vessel tilted at the tilt angle (X°), wherein the tilt angle (X) and the linear velocity (Y) satisfy the following (formula 1): Y≤5.075X+123 (formula 1).


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