The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 06, 2020
Filed:
Jun. 05, 2014
Wuhan Yzy Biopharma Co., Ltd., Wuhan, CN;
Pengfei Zhou, Wuhan, CN;
Jing Zhang, Wuhan, CN;
Rui Wang, Wuhan, CN;
Xiang Zhou, Wuhan, CN;
Xiaoyan Liu, Wuhan, CN;
Lingli Hu, Wuhan, CN;
Sisi Fu, Wuhan, CN;
Hui Zhong, Wuhan, CN;
Wuhan YZY Biopharma Co., Ltd., Wuhan, CN;
Abstract
Provided are compositions and methods for preparing a cell suitable for producing a bispecific antibody. A plurality of eukaryotic cells are incubated with an agent under conditions to allow the cells to arrest at G1/S phase. The agent is then removed from the cells and the cells are transfected with a first vector comprising a sequence encoding a first monovalent antigen-binding unit having specificity to a first antigen and a second vector comprising a sequence encoding a second monovalent antigen-binding unit having specificity to a second antigen. A cell is identified from the plurality of cells that expresses both the first and the second antigen-binding units.