The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 2020

Filed:

Aug. 03, 2016
Applicants:

Riken, Wako-shi, Saitama, JP;

Zeon Corporation, Tokyo, JP;

The Yokohama Rubber Co., Ltd., Tokyo, JP;

Inventors:

Ryoko Orishimo, Wako, JP;

Tomokazu Shirai, Wako, JP;

Kazuhiro Takahashi, Tokyo, JP;

Misao Hiza, Hiratsuka, JP;

Yusuke Tanabe, Hiratsuka, JP;

Assignees:

RIKEN, Wako-shi, JP;

ZEON CORPORATION, Tokyo, JP;

THE YOKOHAMA RUBBER CO., LTD., Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/00 (2006.01); C12P 7/42 (2006.01); C12N 9/10 (2006.01); C12P 5/02 (2006.01); C12N 9/88 (2006.01); C12N 15/09 (2006.01); C12N 5/10 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 5/026 (2013.01); C12N 5/10 (2013.01); C12N 9/88 (2013.01); C12N 15/09 (2013.01); C12N 15/52 (2013.01); C12P 5/02 (2013.01); C12Y 401/01033 (2013.01);
Abstract

A method which enables olefin compound production with a high productivity and an enzyme used in the method, a mutation involving amino acid substitution has been introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Next, the result of evaluating the variants for the catalytic activity related to the production of olefin compounds such as isoprene has revealed that MVD whose threonine at position 209 is substituted with a different amino acid has the catalytic activity, and that MVD whose arginine at position 74 is further substituted with a different amino acid in addition to position 209 has the catalytic activity at higher levels.


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