The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 08, 2020

Filed:

Oct. 30, 2015
Applicant:

Indiana University Research and Technology Corporation, Indianapolis, IN (US);

Inventors:

Mervin C. Yoder, Indianapolis, IN (US);

David A. Ingram, Indianapolis, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/0789 (2010.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0068 (2013.01); C12N 5/0647 (2013.01); C12N 5/0692 (2013.01); G01N 33/5005 (2013.01); G01N 33/56966 (2013.01); A61K 2035/124 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/22 (2013.01); C12N 2501/26 (2013.01); C12N 2502/28 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01); G01N 2333/70589 (2013.01); G01N 2333/70596 (2013.01);
Abstract

A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential—endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived from cord blood, or from HUVECs and HAECs. EPCs were found to reside in vessel walls. Use of a feeder layer of cells derived from high proliferative potential-endothelial colony forming cells (HPP-ECPCS) from human umbilical cord blood, stimulates growth and survival of repopulating hematopoietic stem and progenitor cells. Stimulation of growth and survival was determined by increased numbers of progenitor cells in in vitro cultures and increased levels of human cell engraftment in the NOD/SCID immunodeficient mouse transplant system.


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