The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2020

Filed:

Apr. 04, 2018
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

Srivatsan Raman, Middleton, WI (US);

Aseem Zoe Ansari, Madison, WI (US);

Xiangyang Liu, Madison, WI (US);

Jose Arcadio Rodriguez-Martinez, Dorado, PR (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12N 15/1051 (2013.01); C12N 15/1058 (2013.01); C12N 15/1086 (2013.01);
Abstract

Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.


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