The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2020
Filed:
Mar. 24, 2016
Sigma-aldrich Co. Llc, St Louis, MO (US);
Kenneth B Heuermann, St Louis, MO (US);
Carol A Kreader, St Louis, MO (US);
Jaime K Robert, St Louis, MO (US);
Brian W Ward, St. Louis, MO (US);
Sigma-Aldrich Co. LLC, St. Louis, MO (US);
Abstract
Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3' sequence having complementarity to a portion of the RNA molecule, a non-complementary 5′ sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.