The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2020

Filed:

Sep. 29, 2015
Applicant:

Tokyo Electron Limited, Tokyo, JP;

Inventors:

Kunitada Hatabayashi, Tokyo, JP;

Kentaro Tomita, Tokyo, JP;

Shinichi Gomi, Tokyo, JP;

Tomoaki Kurakazu, Stevenage, GB;

Yasuhiro Oshima, Stevenage, GB;

Kenichi Kagawa, Tokyo, JP;

Shigenori Ozaki, Tokyo, JP;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12Q 1/02 (2013.01); C12M 23/22 (2013.01); C12M 29/00 (2013.01); C12M 33/00 (2013.01); C12M 41/36 (2013.01); C12M 41/46 (2013.01); C12M 41/48 (2013.01); C12N 5/0081 (2013.01); C12N 5/0696 (2013.01); G01N 33/5073 (2013.01); C12N 2500/32 (2013.01); G01N 2333/47 (2013.01);
Abstract

There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.


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