The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 30, 2020

Filed:

Oct. 06, 2017
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Bee Luan Khoo, Singapore, SG;

Jongyoon Han, Bedford, MA (US);

Kyungyong Choi, Cambridge, MA (US);

Hyunryul Ryu, Boston, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/574 (2006.01); B01L 3/00 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
G01N 33/574 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); G01N 33/5073 (2013.01); G01N 33/56966 (2013.01); G01N 33/57488 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/088 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0409 (2013.01);
Abstract

This invention describes a one-step technique for the simultaneous label-free detection and concentration of blast cells from a blood sample. Enrichment of blast cells is achieved using a closed loop microfluidics system, allowing continuous removal of waste and non-target cells to generate concentrated samples of high purity without the need for specific targeting of proteins by antibodies. The technique is highly effective for samples which cannot be purified in a single run. The application of detecting rare blast cells for monitoring minimal residual disease in leukemia patients is demonstrated. The sensitivity of the invention promotes the detection of blast cells in blood samples of early-stage patients.


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