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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10682640 B1

PDFFull Text
Date of Patent:
Jun. 16, 2020

Filed:

Apr. 28, 2017

Anionic exchange-hydrophobic mixed mode

Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Jiali Liao, Hercules, CA (US);

Russell Frost, Hercules, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:

Kilpatrick Townsend and Stockton LLP

Primary Examiner:

Kara M Peo

Int. Cl.
CPC ...
B01J 41/20 (2006.01); C07K 1/16 (2006.01); B01J 20/22 (2006.01); B01D 15/30 (2006.01); B01J 20/281 (2006.01); B01D 15/26 (2006.01); C07K 1/14 (2006.01); B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); B01J 20/286 (2006.01); B01J 20/289 (2006.01); B01J 20/32 (2006.01); B01J 41/09 (2017.01); B01J 20/26 (2006.01); B01J 20/28 (2006.01); B01J 20/288 (2006.01); B01J 41/04 (2017.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
B01J 41/20 (2013.01); B01D 15/265 (2013.01); B01D 15/30 (2013.01); B01D 15/327 (2013.01); B01D 15/363 (2013.01); B01D 15/3847 (2013.01); B01J 20/22 (2013.01); B01J 20/267 (2013.01); B01J 20/281 (2013.01); B01J 20/286 (2013.01); B01J 20/288 (2013.01); B01J 20/289 (2013.01); B01J 20/28016 (2013.01); B01J 20/3208 (2013.01); B01J 20/3217 (2013.01); B01J 20/3219 (2013.01); B01J 20/3253 (2013.01); B01J 20/3265 (2013.01); B01J 20/3293 (2013.01); B01J 41/04 (2013.01); B01J 41/09 (2017.01); C07K 1/14 (2013.01); C07K 1/16 (2013.01); C07K 1/165 (2013.01); C07K 16/00 (2013.01); G01N 30/482 (2013.01); B01J 2220/52 (2013.01);
Abstract

Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)-R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

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