The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 2020
Filed:
Sep. 06, 2013
Dow Agrosciences Llc, Indianapolis, IN (US);
Noel Cogan, Macleod, AU;
John Forster, Diamond Creek, AU;
Matthew Hayden, Templestowe, AU;
Tim Sawbridge, Coburg, AU;
German Spangenberg, Bundoora, AU;
Steven R. Webb, Westfield, IN (US);
Manju Gupta, Carmel, IN (US);
W. Mike Ainley, Carmel, IN (US);
Matthew J. Henry, Indianapolis, IN (US);
John Mason, Preston, AU;
Sandeep Kumar, Carmel, IN (US);
Stephen Novak, Westfield, IN (US);
Dow AgroSciences LLC, Indianapolis, IN (US);
Abstract
An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.