The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 05, 2020

Filed:

Apr. 05, 2017
Applicant:

Nantong University, Nantong, Jiangsu, CN;

Inventors:

Xiaosong Gu, Jiangsu, CN;

Fei Ding, Jiangsu, CN;

Xin Tang, Jiangsu, CN;

Yumin Yang, Jiangsu, CN;

Bin Yu, Jiangsu, CN;

Shiying Li, Jiangsu, CN;

Songlin Zhou, Jiangsu, CN;

Luzhong Zhang, Jiangsu, CN;

Yaxian Wang, Jiangsu, CN;

Yun Gu, Jiangsu, CN;

Hualin Sun, Jiangsu, CN;

Assignee:

NANTONG UNIVERSITY, Nantong, Jiangsu, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); A61L 27/36 (2006.01); A61L 27/56 (2006.01); A61L 27/54 (2006.01); A61L 27/24 (2006.01); A61L 27/22 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
A61L 27/3675 (2013.01); A61L 27/22 (2013.01); A61L 27/24 (2013.01); A61L 27/3687 (2013.01); A61L 27/54 (2013.01); A61L 27/56 (2013.01); C12N 15/113 (2013.01); A61L 2300/412 (2013.01); A61L 2430/32 (2013.01); C12N 2310/113 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01);
Abstract

Provided is a use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. The regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral nerve injury.


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