The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 28, 2020
Filed:
Aug. 27, 2018
Jiangnan University, Wuxi, CN;
Long Liu, Wuxi, CN;
Jian Chen, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Yaokang Wu, Wuxi, CN;
Taichi Chen, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, Jiangsu, CN;
Abstract
The present invention discloses a method for producing N-acetylglucosamine (GlcNAc) by co-utilizing glucose and xylose based on CRISPRi, and belongs to the field of genetic engineering. According to the method,BSGNY-P-glmS-P-GNA1 is used as an original strain, dCas9 induced by xylose and three sgRNA expression fragments targeting to genes zwf, pfkA and glmM respectively are integrated on the genome, and the strain is fermented in a shake flask, so that the titer of GlcNAc reaches 20.5 g/L, the yield of GlcNAc is 0.612 g/g glucose, at the same time, the efficient co-utilizing of glucose and xylose by the recombinantis achieved, and the foundation for further metabolic engineering transformation of theto produce GlcNAc and industrialization thereof is laid.