The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 28, 2020
Filed:
Dec. 22, 2016
Max-planck-gesellschaft Zur Forderung Der Wissenschaften E.v., Munich, DE;
Massachusetts Institute of Technology, Cambridge, MA (US);
Whitehead Institute for Biomedical Research, Cambridge, MA (US);
University of Massachusetts, Boston, MA (US);
Thomas Tuschl, Brooklyn, NY (US);
Sayda Mahgoub Elbashir, Cambridge, MA (US);
Winfried Lendeckel, Hohengandern, DE;
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V., Munich, DE;
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);
Whitehead Institute for Biomedical Research, Cambridge, MA (US);
UNIVERSITY OF MASSACHUSETTS, Boston, MA (US);
Abstract
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using ain vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.