The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2020

Filed:

Oct. 28, 2016
Applicant:

Medimmune Limited, Cambridge, GB;

Inventors:

Nicholas J. Bond, Cambridge, GB;

Suzanne J. Gibson, Cambridge, GB;

Diane Hatton, Cambridge, GB;

Daniel R. Higazi, Cambridge, GB;

Sarah Milne, Cambridge, GB;

Mariana Alessia Sheriff, Cambridge, GB;

Assignee:

MedImmune Limited, Cambridge, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/00 (2006.01); G01N 33/68 (2006.01); C07K 16/18 (2006.01);
U.S. Cl.
CPC ...
C07K 16/00 (2013.01); C07K 16/18 (2013.01); C07K 2317/515 (2013.01); C07K 2317/94 (2013.01); C07K 2319/02 (2013.01);
Abstract

We discovered that recombinant antibody light chains having a murine secretory leader sequence and an SYE motif at the N-terminus are truncated during post-translational processing. This disclosure provides two protein engineering solutions: to alter the SYE amino acid sequence of the Lc N-terminus to other alternatives; or to change the secretory leader peptide sequence. We have shown that both of these solutions are effective for preventing N-terminal light chain truncation.


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