The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 07, 2020

Filed:

Jun. 23, 2015
Applicant:

International Business Machines Corporation, Armonk, NY (US);

Inventors:

Gustavo A. Stolovitzky, Riverdale, NY (US);

George F. Walker, New York, NY (US);

Chao Wang, Ossining, NY (US);

Deqiang Wang, Ossining, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); C12N 15/10 (2006.01); G01N 27/447 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 15/101 (2013.01); G01N 27/44747 (2013.01); G01N 27/44791 (2013.01);
Abstract

A mechanism is provided for reducing entropy of a polyelectrolyte before the polyelectrolyte moves through a nanopore. A free-standing membrane has the nanopore formed through the membrane. An agarose gel is formed onto either and/or both sides of the nanopore in the membrane. The agarose gel is a porous material. The polyelectrolyte is uncoiled by driving the polyelectrolyte through the porous material of the agarose gel via an electric field. Driving the polyelectrolyte, having been uncoiled and linearized by the agarose gel, into the nanopore is for sequencing.


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