The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 07, 2020

Filed:

Apr. 20, 2018
Applicant:

Ajinomoto Co., Inc., Tokyo, JP;

Inventors:

Shunsuke Yamazaki, Kanagawa, JP;

Tomoko Shimizu, Kanagawa, JP;

Kenichi Mori, Kanagawa, JP;

Naoto Tonouchi, Kanagawa, JP;

Assignee:

AJINOMOTO CO., INC., Tokyo, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/245 (2006.01); C08B 37/00 (2006.01); C12N 15/70 (2006.01); C12P 19/26 (2006.01); C12P 19/04 (2006.01);
U.S. Cl.
CPC ...
C07K 14/245 (2013.01); C08B 37/0075 (2013.01); C12N 15/70 (2013.01); C12P 19/04 (2013.01); C12P 19/26 (2013.01);
Abstract

A method for producing heparosan is provided. Heparosan is produced by culturing anbacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybil, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.


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