The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2020
Filed:
Aug. 28, 2015
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Gregory Lohman, Cambridge, MA (US);
Thomas C. Evans, Jr., Topsfield, MA (US);
Larry A. McReynolds, Beverly, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6862 (2018.01); C12Q 1/6853 (2018.01); C12N 9/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6862 (2013.01); C12N 9/93 (2013.01); C12Q 1/6853 (2013.01); C12Y 605/01003 (2013.01); G01N 2333/9015 (2013.01);
Abstract
Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.