The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 10, 2020
Filed:
Jun. 02, 2017
Applicant:
Takara Bio Usa, Inc., Mountain View, CA (US);
Inventors:
Hiroyuki Matsumoto, Mountain View, CA (US);
Michael Haugwitz, Belmont, CA (US);
Andrew Farmer, Los Altos, CA (US);
Magnolia Bostick, San Mateo, CA (US);
Assignee:
Takara Bio USA, Inc., Mountain View, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6876 (2018.01); C12N 15/90 (2006.01); C12Q 1/6806 (2018.01); C12N 15/66 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 15/10 (2013.01); C12N 15/66 (2013.01); C12N 15/907 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6876 (2013.01); C12Q 2600/156 (2013.01);
Abstract
Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention.