The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 18, 2020
Filed:
Sep. 10, 2015
Fundació Institut DE Ciències Fotòniques, Castelldefels-Barcelona, ES;
Fundació Centre DE Regulació Genòmica, Barcelona, ES;
Institució Catalana DE Recerca I Estudis Avançats, Barcelona, ES;
Melike Lakadamyali, Castelldefels-Barcelona, ES;
Carlo Manzo, Castelldefels-Barcelona, ES;
Maria Aurelia Ricci, Barcelona, ES;
Maria Pia Cosma, Barcelona, ES;
FUNDACIÓ INSTITUT DE CIÈNCIES FOTÓNEQUES, Castelldefels-Barcelona, ES;
FUNDACIÓ CENTRE DE REGULACIÓ GENÒMICA, Barcelona, ES;
INSTITUCIÓ CATALANA DE RECERCA↑ESTUDIS AVANçATS, Barcelona, ES;
Abstract
The present invention relates to methods for detecting the chromatin state of a cell based on recording a super resolution image of nucleosome organization and correlating said imaged with size of nucleosomal clutches, nucleosomal density and/or number of nucleosomes per nucleosomal clutches. Additionally, the invention relates to a kit comprising a first antibody capable of specifically binding to a histone protein and a photoswitchable fluorophore linked-secondary antibody and the use of the kit of the invention for detecting the chromatin state of a cell and isolating a cell in an open chromatin state or in a closed chromatin state. The invention also relates to a device adapted to detect the chromatin state of a cell.