The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 18, 2020
Filed:
Sep. 20, 2017
Applicant:
Northwestern University, Evanston, IL (US);
Inventors:
Milan Mrksich, Hinsdale, IL (US);
Maria D. Cebezas, Evanston, IL (US);
Chad A. Mirkin, Wilmette, IL (US);
Assignee:
NORTHWESTERN UNIVERSITY, Evanston, IL (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/42 (2006.01); C12Q 1/37 (2006.01); C12Q 1/34 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/42 (2013.01); C12Q 1/34 (2013.01); C12Q 1/37 (2013.01); C12Y 304/22044 (2013.01); C12Y 305/01098 (2013.01); G01N 2333/916 (2013.01); G01N 2333/95 (2013.01); G01N 2333/98 (2013.01); G01N 2560/00 (2013.01); G01N 2610/00 (2013.01);
Abstract
The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.